Table 2.

List of miRs differentially expressed (p<0.05) on screening microarray, and confirmation of expression by individual Taqman Real-Time PCR with pre-amplification.Fold change refers to Recovered (R)/Dependent (D) ratio. A negative number indicates lower expression in the Recovered group compared with Dependent. Of the 10 miRs found differentially expressed by screening array, miRs-15b, -23a, -26a, and -195 showed decreased expression by confirmatory PCR. Of these 4, miRs-23a and -195 were also differentially expressed in the Validation Cohort. In the Test Cohort N= 6 each R and D for the Screening Array and N=7 each R and D for the Confirmatory PCR.

miR Expression
Screening Microarray (Test Cohort)			Confirmatory PCR (Test Cohort)			Confirmatory PCR (Validation Cohort)
miR ID	Fold Change	p -Value	miR ID	Fold Change	p -Value	miR ID	Fold Change	p -Value
	R/D			R/D			R/D
miR-181b	11.38	0.002	miR-181b*	N/A	N/A	miR-181b*	No difference	NS
miR-424	−4.38	0.027	miR-424	No difference	NS	miR-424	N/A	N/A
miR-376a	5.41	0.028	miR-376a	No difference	NS	miR-376a	N/A	N/A
miR-195	−2.91	0.029	miR-195	−1.8	0.01	miR-195	−1.5	0.03
miR-27a	−4.18	0.032	miR-27a	No difference	NS	miR-27a	N/A	NS
miR-23a	−4.52	0.032	miR-23a	−2.2	0.03	miR-23a	−1.8	0.01
miR-103	3.69	0.044	miR-103	No difference	NS	miR-103	N/A	N/A
miR-26a	−14.92	0.044	miR-26a	−1.4	0.04	miR-26a	No difference	NS
miR-142-3p	6.35	0.048	miR-142-3p	No difference	NS	miR-142-3p	N/A	N/A
miR-15b	−12.73	0.048	miR-15b	−1.5	0.04	miR-15b	−1.8	0.09

^*miR-181b could not be validated by this method in the Test Cohort, but was not significantly altered in the Validation cohort.

NS, not significant;

N/A: not applicable. These measurements were not performed.