Table 2.
List of miRs differentially expressed (p<0.05) on screening microarray, and confirmation of expression by individual Taqman Real-Time PCR with pre-amplification.Fold change refers to Recovered (R)/Dependent (D) ratio. A negative number indicates lower expression in the Recovered group compared with Dependent. Of the 10 miRs found differentially expressed by screening array, miRs-15b, -23a, -26a, and -195 showed decreased expression by confirmatory PCR. Of these 4, miRs-23a and -195 were also differentially expressed in the Validation Cohort. In the Test Cohort N= 6 each R and D for the Screening Array and N=7 each R and D for the Confirmatory PCR.
miR Expression
| ||||||||
---|---|---|---|---|---|---|---|---|
Screening Microarray (Test Cohort) | Confirmatory PCR (Test Cohort) | Confirmatory PCR (Validation Cohort) | ||||||
miR ID | Fold Change | p -Value | miR ID | Fold Change | p -Value | miR ID | Fold Change | p -Value |
R/D | R/D | R/D | ||||||
miR-181b | 11.38 | 0.002 | miR-181b* | N/A | N/A | miR-181b* | No difference | NS |
miR-424 | −4.38 | 0.027 | miR-424 | No difference | NS | miR-424 | N/A | N/A |
miR-376a | 5.41 | 0.028 | miR-376a | No difference | NS | miR-376a | N/A | N/A |
miR-195 | −2.91 | 0.029 | miR-195 | −1.8 | 0.01 | miR-195 | −1.5 | 0.03 |
miR-27a | −4.18 | 0.032 | miR-27a | No difference | NS | miR-27a | N/A | NS |
miR-23a | −4.52 | 0.032 | miR-23a | −2.2 | 0.03 | miR-23a | −1.8 | 0.01 |
miR-103 | 3.69 | 0.044 | miR-103 | No difference | NS | miR-103 | N/A | N/A |
miR-26a | −14.92 | 0.044 | miR-26a | −1.4 | 0.04 | miR-26a | No difference | NS |
miR-142-3p | 6.35 | 0.048 | miR-142-3p | No difference | NS | miR-142-3p | N/A | N/A |
miR-15b | −12.73 | 0.048 | miR-15b | −1.5 | 0.04 | miR-15b | −1.8 | 0.09 |
miR-181b could not be validated by this method in the Test Cohort, but was not significantly altered in the Validation cohort.
NS, not significant;
N/A: not applicable. These measurements were not performed.