Figure 3. Electrical silencing of PDF neurons disrupts circadian rhythms in a reversible fashion.

(A) Representative actograms of control and treated animals are shown. Flies were raised in standard medium throughout development, and young adults were transferred to test tubes containing RU or vehicle. Flies were entrained for 4 days, spent 8 days in DD (DD_1-8) with or without RU (Veh), following which all experimental groups were transferred back to control medium (Veh) for 10 additional days (DD_9-18). (B) Percentage of rhythmicity for the indicated groups across the different treatments. Data obtained around food transfer, on DD_9-10, were not taken into account for this analysis. Data represents 3 independent experiments and the number of total flies analyzed per treatment ranged from 45 to 64. *** indicates p<0,001 (ANOVA with Bonferroni post hoc test). See also Figure S2.