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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Mol Microbiol. 2011 Oct 12;82(3):679–697. doi: 10.1111/j.1365-2958.2011.07845.x

Figure 9.

Figure 9

Bb CoADR promotes survival of spirochetes within fed, but not flat, nymphs. Ixodes scapularis nymphs were rectally-infused with equivalent densities of wild-type (c162), cdr mutant (c309), and complemented (c1655) clones of B. burgdorferi. (A) After a ten day ‘rest’ period, relative B. burgdorferi burdens in unfed ticks were analyzed by qRT-PCR; the number of copies of the B. burgdorferi flaB transcript were normalized to tick β-actin RNA. The remainder of the rectally-infused nymphs were then allowed to feed upon SCID mice to repletion. (B) B. burgdorferi burdens in fed nymphs were monitored by qRT-PCR. The asterisk (*) denotes a significant difference between spirochetes in the midguts of fed nymphs infused with the cdr mutant compared to those infused with either the wild-type or the complement (P < 0.05). The data represent the average of four replicates of qRT-PCR analysis on two pools of three ticks (unfed) or 1–2 ticks (fed) from a representative experiment.