Table 1.

Spectral properties of quenchers conjugated at the 5’-terminus of 20mer and 15mer oligonucleotides.

	20mer		15mer
Quencher	λmax	Δλ(half max)	λmax	Δλ(half max)
MPQ1	557 nm	481 - 618 (137 nm)	560 nm	481 - 619 (138 nm)
MPQ2	614 nm	522 - 676 (154 nm)	524 nm	446 - 640 (194 nm)
MPQ3	521 nm	461 - 574 (113 nm)	529 nm	478 - 571 (93 nm)
MPQ4	539 nm	467 - 606 (139 nm)	551 nm	469 - 611 (142 nm)
MPQ5	503 nm, 623 nm	447 - 689 (242 nm)	646 nm	524 - 723 (199 nm)
MPQ6	536 nm, 656 nm	463 - 731 (268 nm)	537 nm, 657 nm	463 - 736 (273 nm)
Dabcyl	472 nm	405 - 520 (115 nm)	472 nm	405 - 519 (114 nm)
BHQ2	580 nm	503 - 649 (146 nm)	581 nm	507 - 652 (145 nm)