Table 2.
Contact quenching efficiencies of fluorophore/quencher pairs. Results are provided as the average of three experiments with standard deviation shown. See Fig. 3a for structural context.
| Alexa 350 | FAM | Cy 3 | TAMRA | Atto 590 | Quasar 670 | |
|---|---|---|---|---|---|---|
| Quencher | λem = 442nm | λem = 517nm | λem = 563nm | λem = 580nm | λem = 624nm | λem = 662nm |
| MPQ1 | 73.6 ± 0.7% | 95.4 ± 0.2% | 98.0 ± 0.4% | 86.0 ± 0.7% | 96.3 ± 0.2% | 96.1 ± 0.3% |
| MPQ2 | 72.4 ± 0.1% | 94.9 ± 0.4% | 97.6 ± 0.2% | 87.8 ± 0.4% | 96.1 ± 0.1% | 97.0 ± 0.2% |
| MPQ3 | 74.7 ± 0.9% | 96.5 ± 0.2% | 97.3 ± 0.2% | 86.8 ± 0.5% | 96.6 ± 0.2% | 93.3 ± 0.4% |
| MPQ4 | 72.1 ± 1.2% | 96.0 ± 0.2% | 97.6 ± 0.3% | 88.8 ± 1.0% | 96.2 ± 0.7% | 94.7 ± 0.2% |
| MPQ5 | 69.7 ± 3.5% | 93.7 ± 0.1% | 96.8 ± 0.4% | 85.7 ± 0.4% | 95.1 ± 0.2% | 95.4 ± 0.3% |
| MPQ6 | 71.6 ± 1.8% | 95.2 ± 0.2% | 98.2 ± 0.6% | 87.2 ± 0.2% | 96.4 ± 0.2% | 95.6 ± 0.8% |
| Dabcyl | 75.7 ± 0.7% | 96.0 ± 0.2% | 97.4 ± 0.1% | 87.3 ± 0.6% | 95.7 ± 0.3% | 83.9 ± 0.4% |
| BHQ2 | 77.0 ± 0.7% | 96.5 ± 0.2% | 98.9 ± 0.3% | 87.8 ± 0.2% | 97.4 ± 0.3% | 98.2 ± 0.2% |