Table 3.
Mixed mechanism quenching efficiencies of fluorophore/quencher pairs. Results are provided as the average of three experiments with standard deviation shown. See Fig. 3b for structural context.
| Alexa 350 | FAM | Cy 3 | TAMRA | Atto 590 | Quasar 670 | |
|---|---|---|---|---|---|---|
| Quencher | λem = 442nm | λem = 517nm | λem = 563nm | λem = 580nm | λem = 624nm | λem = 662nm |
| MPQ1 | 66.4 ± 0.9% | 90.7 ± 0.4% | 85.6 ± 0.4% | 79.7 ± 0.7% | 93.6 ± 1.0% | 90.2 ± 0.5% |
| MPQ2 | 66.6 ± 3.1% | 89.1 ± 0.3% | 84.0 ± 0.7% | 79.6 ± 0.2% | 91.3 ± 0.8% | 91.1 ± 0.3% |
| MPQ3 | 66.0 ± 0.1% | 89.1 ± 0.4% | 83.5 ± 1.0% | 77.9 ± 0.3% | 89.5 ± 1.1% | 78.7 ± 0.5% |
| MPQ4 | 62.2 ± 2.5% | 84.1 ± 0.2% | 85.1 ± 0.6% | 79.1 ± 0.9% | 92.6 ± 0.6% | 82.8 ± 1.3% |
| MPQ5 | 58.7 ± 1.2% | 77.4 ± 1.8% | 80.2 ± 0.3% | 75.5 ± 0.6% | 90.0 ± 1.1% | 85.9 ± 0.7% |
| MPQ6 | 64.8 ± 0.8% | 87.8 ± 0.8% | 92.9 ± 0.4% | 80.6 ± 0.3% | 95.2 ± 0.4% | 93.5 ± 0.3% |
| Dabcyl | 70.4 ± 3.2% | 87.1 ± 0.3% | 77.8 ± 0.9% | 64.3 ± 0.7% | 80.7 ± 1.7% | 66.5 ± 3.7% |
| BHQ2 | 71.6 ± 0.8% | 91.9 ± 1.1% | 90.8 ± 0.6% | 81.2 ± 0.3% | 95.4 ± 0.5% | 94.6 ± 0.1% |