. Author manuscript; available in PMC: 2012 Nov 16.

Published in final edited form as: Bioconjug Chem. 2011 Oct 28;22(11):2345–2354. doi: 10.1021/bc200424r

Table 4.

FRET quenching efficiences of fluorophore/quencher pairs. Results are provided as the average of three experiments with standard deviation shown. See Fig. 3c for structural context.

	FAM	Cy 3	TAMRA	Atto 590	Quasar 670
Quencher	λ_em = 517nm	λ_em = 563nm	λ_em = 580nm	λ_em = 624nm	λ_em = 662nm
MPQ1	53.1 ± 1.4%	62.4 ± 0.7%	77.1 ± 0.5%	45.6 ± 1.5%	28.9 ± 0.8%
MPQ2	50.3 ± 0.2%	60.7 ± 2.2%	74.9 ± 1.4%	43.9 ± 0.8%	33.5 ± 1.2%
MPQ3	60.2 ± 1.3%	59.6 ± 0.9%	74.1 ± 0.4%	29.1 ± 0.9%	22.2 ± 0.5%
MPQ4	47.3 ± 1.4%	56.9 ± 1.9%	73.7 ± 0.6%	33.4 ± 2.1%	25.6 ± 2.9%
MPQ6	50.4 ± 0.9%	64.4 ± 1.2%	78.6 ± 0.4%	57.6 ± 0.6%	43.0 ± 0.5%
Dabcyl	41.3 ± 0.9%	52.1 ± 2.8%	71.1 ± 0.8%	26.8 ± 1.9%	22.5 ± 3.1%
BHQ2	54.3 ± 1.0%	63.5 ± 1.8%	80.4 ± 1.2%	60.0 ± 1.3%	40.4 ± 2.8%
No Quencher	29.2 ± 0.7%	53.2 ± 1.2%	71.4 ± 1.1%	23.3 ± 1.4%	23.9 ± 1.5%