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. 2001 Apr;12(4):809–820. doi: 10.1091/mbc.12.4.809

Figure 3.

Figure 3

Binding of beads coated with mADAM 3 disintegrin domains: role of the disintegrin loop. Fluorescent beads were coated with mADAM 3 disintegrin domains prepared, as indicated, in either Drosophila cells (D-ADAM 3) or E. coli (E-ADAM 3) and then processed for binding to zona-free murine eggs as described in MATERIALS AND METHODS. (A). Eggs were preincubated for 30 min at 37°C with either a scrambled (scr.) or an authentic (loop) mADAM 3 disintegrin loop peptide at a final concentration of 1 mM peptide. (B) Beads were coated as indicated with either wild-type or the D6A/Q7A mADAM 3 disintegrin domain and then processed for binding to eggs. (C) Eggs were preincubated for 30 min at 37°C with, as indicated, either the D6A/Q7A mutant (mut.) or the wild-type (wt) mADAM 3 disintegrin domain (20 μM), and then processed for binding of beads coated with wt-mADAM 3 disintegrin domain.