Figure 4.

Sequence-specific activity of the mADAM 2 disintegrin domain. Zona-free eggs were preincubated for 30 min with GST-ADAM 2 or the indicated GST-ADAM 2 mutant disintegrin domain at a final concentration of 1 μM. Sperm-egg binding was then assayed as described in the legend to Figure 2. Data represent the average percentage of inhibitory activity of wild-type and mutant mADAM 2 disintegrin domains (compared with buffer controls). The data were compiled from three or four independent experiments. Bars represent the SE of the mean. Data for the R2A, D6A, E7A, D9A, and E12A mutants are from Bigler et al. (2000). Disintegrin domains compared within an individual experiment were prepared on the same day.