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. 2001 Apr;12(4):809–820. doi: 10.1091/mbc.12.4.809

Figure 8.

Figure 8

Effects of antibodies to β1 integrin-associated proteins on fertilization and ADAM 3 bead binding. (A) Zona-free eggs were processed for immunofluorescence with mAbs against CD81 and CD98 as described in MATERIALS AND METHODS. The control antibody for CD81 was hamster IgG and that for CD98 was rat IgG. Sperm-egg binding (B) and fusion (C) were assayed following preincubation with 200 μg/ml the indicated antibodies as described in the legend to Figure 2. The data in B and C are from one experiment. The experiment was repeated three times with similar results. (D) Fluorescent beads coated with E. coli mADAM 3 disintegrin domain were processed for binding to zona-free eggs following a 30-min preincubation with 200 μg/ml the indicated antibodies as described in MATERIALS AND METHODS. The sample labeled control was preincubated with buffer. Scion image analysis (graph to the right in D) was performed as described in the legend to Figure 6. The anti-CD81 antibody used in the experiments shown was the mAb 2F7.