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. Author manuscript; available in PMC: 2012 Nov 9.
Published in final edited form as: J Am Chem Soc. 2011 Oct 19;133(44):17855–17868. doi: 10.1021/ja207220g

Figure 6.

Figure 6

Overlay of 1H-15N TROSY spectra of the middle Ub in all-natural homogeneously K11-linked Ub3 (red) and in blue, (a) WT Ub, (b) the distal Ub of K11-linked Ub2, and (c) the proximal Ub of K11-linked Ub2. The spectral differences between residues in the middle Ub unit of K11-linked Ub3 and (d) WT Ub, (e) distal Ub of K11-linked Ub2, and (f) proximal Ub of K11-linked Ub2 are quantified as amide chemical shift perturbations (CSPs). Residues with significant CSPs are indicated on the spectra in panels a–c.