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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Biochem J. 2011 Dec 15;440(3):385–395. doi: 10.1042/BJ20102148

Figure 2. Adiponectin blocks leptin-stimulated Ob-Rb phosphorylation.

Figure 2

A) Top Panel: Western blots of phospho-Ob-Rb[Tyr985] in lysates prepared from rat HSCs. Thirty-minute leptin treatment (Lep, 100ng/ml) increased Jak2 (Tyr985) phosphorylation significantly (*p < 0.05) compared to untreated samples (Utx). Co-administration of leptin (100ng/ml) and adiponectin (10μg/ml) (LA) inhibited leptin-stimulated Ob-Rb(Tyr985) phosphorylation (#p < 0.05 vs. leptin). Bottom Panel: Quantitation of data presented above. B) Top Panel: Western Blots of phospho-Ob-Rb (Tyr985) from liver tissue collected from Ad−/− mice and wild-type (WT) mice. Ob-Rb phosphorylation was significantly increased in CCl4 + leptin-treated Ad−/− mice, compared to similarly treated wild-type mice (*p < 0.05 vs. WT). Bottom Panel: Quantitation of data presented (2A) and (2B).