Figure 5.

mtDNA depletion in lopo¹. A quantitative Southern blot was generated containing equal amounts of total DNA from wild-type Canton S (wt) and lopo¹ (lopo) digested with EcoRI (equivalent of 100 mg of third instar larvae). mtDNA was visualized using a 5-kb fragment of mtDNA (Garesse, 1988). No mtDNA is detected in lopo¹. Efficiency of loading and blotting was verified by hybridizing the blot with a single-copy nuclear gene (aos cDNA; Kretzschmar et al., 1992). The corresponding 1.2-kb fragment (and a weaker 1.6-kb fragment) show equal intensities in both lanes.