Larval expression and fractionation of
Drosophila mtSSB protein. Protein
fractions as described in MATERIALS AND METHODS were denatured and
electrophoresed in a 17% SDS-polyacrylamide gel, transferred to
nitrocellulose, and detected by immunoblotting with
rabbit antiserum against D. melanogaster mtSSB at a
1:1000 dilution using the ECL method (Amersham Life Sciences, Arlington
Heights, IL). Lane 1, recombinant Drosophila
mtSSB (10 ng); lanes 2–4, Blue Sepharose eluates (60 μl) from
wild-type, heterozygous, and homozygous
lopo1 larvae, respectively; lanes 5–7,
larval extracts (∼200 μg of total protein) from wild-type,
heterozygous, and homozygous lopo larvae,
respectively.