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. 2001 Apr;12(4):821–830. doi: 10.1091/mbc.12.4.821

Figure 6.

Figure 6

Larval expression and fractionation of Drosophila mtSSB protein. Protein fractions as described in MATERIALS AND METHODS were denatured and electrophoresed in a 17% SDS-polyacrylamide gel, transferred to nitrocellulose, and detected by immunoblotting with rabbit antiserum against D. melanogaster mtSSB at a 1:1000 dilution using the ECL method (Amersham Life Sciences, Arlington Heights, IL). Lane 1, recombinant Drosophila mtSSB (10 ng); lanes 2–4, Blue Sepharose eluates (60 μl) from wild-type, heterozygous, and homozygous lopo1 larvae, respectively; lanes 5–7, larval extracts (∼200 μg of total protein) from wild-type, heterozygous, and homozygous lopo larvae, respectively.