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. Author manuscript; available in PMC: 2012 Dec 18.
Published in final edited form as: Toxicology. 2011 Oct 14;290(2-3):279–286. doi: 10.1016/j.tox.2011.10.005

Fig. 1. PMN accumulation and activation in rat livers.

Fig. 1

Rats were treated with two administrations of SLD (50 mg/kg, p.o.) or its vehicle (Veh, 0.5% methyl cellulose) with a 16 hr interval. LPS (8.25 × 105 EU/kg, i.v.) or its saline vehicle was administered half an hour before the second administration of SLD. PMN staining was performed on livers collected 4 hr after the second administration of SLD. (A) PMN numbers in 400 X high power fields (HPF) were counted to evaluate PMN accumulation. (B) HOCl-protein adduct staining was performed on slides of frozen liver collected at 8 hr. Ten randomly chosen fields were photographed for each section, and the fraction of positive pixels was determined. *significantly different from respective group without LPS. #significantly different from Veh/LPS group. P<0.05, n=4-5.