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. 2011 Dec;138(6):641–649. doi: 10.1085/jgp.201110723

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© 2011 Maksaev and Haswell

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Figure 1. — Suitability of Xenopus oocytes for the expression of bacterial mechanosensitive channel MscS. (A) Inactivation of endogenous mechanosensitive channels upon patch excision. Traces from the same water-injected oocyte are shown in cell-attached configuration (top) and excised-patch (bottom). Pipettes with BN 7 at a potential of +40 mV were used. (B) Confocal scan showing a portion of an oocyte expressing MscS-GFP 4 d after injection. Bright field and GFP signal (in green) are superimposed. (C) MscS activation by negative and positive pressure in the pipette, as recorded from the same excised patch in symmetric ND96 buffer. Pipette potential was +30 mV with BN 6.