Table I.
Constructs | [Ca2+] | Slope parameter Δα (mean ± SD) | No. of patches |
M | mmHg−1 | ||
WT | 10−3 | 0.015 ± 0.0043 | 4 |
WT | 10−5 | 0.016 ± 0.0050 | 6 |
WT | 10−6 | 0.018 ± 0.0031 | 4 |
358–486-12GGS | 10−5 | 0.012 ± 0.0033 | 4 |
The ensemble current plots of Po versus pressure from wild type at different [Ca2+] (10−6, 10−5, and 10−3 M) and 358–486-12GGS at intermediate [Ca2+] (10−5 M) were fitted with the Boltzmann equation: Po = 1/[1 + a × exp(−Δα × P)] (Fig. 1 B). The slope parameters, Δα, of the wild type at different [Ca2+] are not significantly different (P = 0.38, 0.28, and 0.28 in a pairwise t test). Δα of 358–456-12GGS is also not significantly different from that of the wild type at 10−5 M Ca2+ (P = 0.07).