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Adhesion quantification of S-phase HOBs and characterization of experimental substrates. (a–c) Adhesion-associated vinculin was immunolabelled, images were scored with a straight white line, and analysed by image analysis software. Substrates were 330 nm deep and had widths of (d) 100 μm, (e) 25 μm and (f) 10 μm.
