Figure 5. Flavonoid induced cell death does not require caspases.
(A) RT-PCR for expression of Caspase-3, Caspase-7, and β-Actin from MEF 119 (Caspase 3 −/−/Caspase7 −/−) and MEF 122 (Caspase 3+/−/Caspase 7+/−, wild type) cells. (B) Cellular number as a percent of DMSO-treated cells after 72 hours of treatment with flavonoids as indicated. Trypan blue exclusion assays were performed as described. Error bars represent standard deviation from three independent experiments. *p<0.05, t-test.