Table 1.
Dye coupling is inhibited in monolayers and cysts of bEnd.3 cells expressing dominant negative Cx
| Clone | Group | Cell-to-cell coupling | SEM | Number of injections |
|---|---|---|---|---|
| Monolayersa | ||||
| bEnd.3 | Control | 9.1 | 0.6 | 15 |
| A3 | Hygromycin | 9.2 | 0.5 | 10 |
| B3 | 3243H7 | 4.0b | 0.5 | 10 |
| B5 | 3243H7 | 2.4b | 0.4 | 10 |
| D1 | Cx43-βGal | 3.9b | 0.6 | 10 |
| D2 | Cx43-βGal | 2.3b | 0.6 | 10 |
| Cystsc | ||||
| bEnd.3 | Control | 8.0 | 0.7 | 4 |
| B5 | 3243H7 | 2.0d | 0.5 | 5 |
| D2 | Cx43-βGal | 1.8d | 0.3 | 4 |
The number of cells labeled with Lucifer Yellow after a 5 min microinjection was determined in subconfluent monolayer cultures of the parental bEnd.3 cell line, a clone transfected with the hygromycin resistance gene only (A3), two independent clones of bEnd.3 cells transfected with either 3243H7 cDNA (B3 and B5) or Cx43βGal cDNA (D1 and D2).
Dye coupling of clones B3, B5, D1, and D2 was significantly different (p < 0.01) from that of both bEnd.3 and A3 cells.
The number of cells labeled with Lucifer Yellow after a 5-min microinjection was determined in cysts grown of the parental bEnd.3 cells, clone B5, or clone D2.
Dye coupling in cysts of clones B5 and D2 was significantly different (p < 0.01) from that in cysts formed by bEnd.3 cells.