Figure 1. AZD8055 is a potent inhibitor of mTORC1 and mTORC2 signaling.
(A) Immunoblot analysis was performed on mTORC1 and mTORC2 effectors after treatment with increasing concentrations of AZD8055 or rapamycin for four hours in MCF-7, BT-474 and MDA-MB-468 breast cancer cells. (B) The same cell lines were treated with 500nM of AZD8055 and collected at indicated times and analyzed by immunoblotting.