Figure 4. mTOR kinase inhibition leads to RTK activation.

(A) BT-474 cells were treated for four hours with either DMSO, 500nM of AZD8055 and phosphorylated levels of different RTKs were assessed by immunoblotting cell lysates applied directly to the AB-bound membranes (R&D systems). Each spot (in duplicate) corresponds to a specific P-RTK (see also Figure S3). (B) Immunoblots analysis were 28 performed on the EGF receptor family members and IGF1-R/IR in BT-474 cells and (C) in MDA-MB-468 cells after treatment with 500nM of AZD8055.