| pRL3 | 4 μg |
| 10xbuffer | 10 μl |
| PstI (10 U/μl) | 4 μl |
| dH2O | ~ |
| Total: | 100 μL |
| Ppa-egl-4p::gfp | 4 μg |
| 10x buffer | 10 μl |
| SalI (10 U/μl) | 4 μl |
| dH2O | ~ |
| Total: | 100 μl |
| gDNA PS312 | 10 μg |
| 10x buffer | 10 μl |
| Pst I and Sal I (10 U/μl) | 8 μl |
| dH2O | ~ |
| Total: | 100 μl |
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