Figure 8.

Summary of the temporal changes in the four Aβ brain pools. a, Decreases with age in all three in vivo ISF Aβ peptides measured by 6E10 Aβ triplex sandwich ELISA (for details, see Fig. 3). b, The fold-decrease measured by ELISA (significant between ages 3 and 24 months by two-way ANOVA; see Fig. 2) is comparable to the fold-decrease measured by IP/WB (though the latter was not significant by one-way ANOVA; for details, see Fig. 4). The total amount measured by IP/WB analysis method was only ∼30% of the total amount measured by ELISA. c, IP/WB analysis of Aβ in brain tissue. By ∼24 months, there was a steep increase in insoluble (SDS- and FA-extracted) Aβ. The TBS-extracted Aβ from the same mice did not rise until 24 months and then only very slightly. Values are means ± SEM from Figures 3 and 4.