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. 2001 Apr;12(4):881–889. doi: 10.1091/mbc.12.4.881

Figure 1.

Figure 1

Mutations in the transmembrane region increase the proportion of CtmPrP, and reveal that this form is slightly larger than SecPrP. mRNA encoding wild-type (WT), A116V, or 3AV PrP was translated in rabbit reticulocyte lysate supplemented with canine pancreatic microsomes. Aliquots of the reaction were then incubated with (lanes 2, 3, 5, 6, 8, and 9) or without (lanes 1, 4, and 7) PK in the presence (lanes 3, 6, and 9) or absence (lanes 1, 2, 4, 5, 7, and 8) of Triton X-100 (Det). Samples were then analyzed by SDS-PAGE and autoradiography. Note the presence of a closely spaced doublet of glycosylated PrP in lanes 1, 4, and 7, corresponding to SecPrP (white arrowheads) and CtmPrP (shaded arrowheads). The protease-protected forms of SecPrP and CtmPrP are indicated by the white and shaded arrows, respectively, in lanes 2, 5, and 8. Molecular size markers are given in kilodaltons.