Figure 10.

Inhibition of endogenous GAG sulfation does not affect the toxicity of Δ105–125 PrP. A, HEK cells expressing WT or Δ105–125 PrP were incubated for 24 h in the presence or absence of 30 mm sodium chlorate (ClO₃) or 100 μg/ml PS, after which incubation was continued for an additional 30 min in the presence or absence of Zeocin (400 μg/ml). Cells were then lysed and analyzed for phosphorylated H2AX (γ-H2AX) and actin by Western blotting. The positions of γ-H2AX, ubiquitinated γ-H2AX (Ub-γ-H2AX), and actin are indicated. B, Western blot signals for Ub-γ-H2AX were quantitated and normalized to the amount of actin. Ub-γ-H2AX levels in the presence of Zeocin were expressed as a fold change relative to levels in the absence of Zeocin. A single experiment, representative of at least three similar ones, is shown.