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. Author manuscript; available in PMC: 2011 Nov 30.
Published in final edited form as: Biochemistry. 2007 Dec 23;47(3):986–996. doi: 10.1021/bi7021624

Figure 9.

Figure 9

S100A4 is activated during cell retraction. The panels show different time points (hours:minutes) during random cell movement over fibronectin. Upper panels: DIC. Lower panels: ratio of Mero over FITC signal. An area of high activation is prominent during retraction. The area of elevated activation persists after the start of a new protrusion in the same area (arrowhead). Elevated Mero-S100A4 ratios were observed in cellular retractions in 5 cells examined. Scale bar = 10 μm. The ratio image was scaled by setting the lower and upper threshold levels to exclude the lower 7% and upper 3%, respectively, and a linear pseudocolor lookup table was applied where the upper threshold limit value was divided by the lower threshold limit value to show the dynamic range of ratio signal.