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. 2001 Apr;12(4):901–917. doi: 10.1091/mbc.12.4.901

Figure 1.

Figure 1

The spo20-KC104 mutant is defective in sporulation. (A) Kinetics of meiosis in a wild-type (WT) and a spo20-KC104 mutant. YN13–14 (h+/h- spo20-KC104/spo20-KC104 ade6-M210/ade6-M216) and YN49–53 (h+/h- ade6-M210/ade6-M216) precultured overnight in liquid growth medium (SSL+N) were incubated with shaking at 25°C in liquid sporulation medium (SSL-N). A portion of the culture was stained with DAPI. Meiotic cells were classified by the number of nuclei per cell. For each sample, ∼500 cells were counted. This figure is depicted on the basis of one representative result of four independent experiments that provided similar results. Diamonds, mononucleate cells; circles, binucleate zygotes; triangles, trinucleate or tetranucleate cells. (B) DAPI image of spo20-KC104 during sporulation. YN10 (spo20-KC104) and YN12 (WT) were incubated at 25°C in sporulation medium (MEA) for 24 h. Chromosomal DNA was stained with DAPI. Bar, 10 μm.