Figure 2.

The spo20-KC104 mutant exhibits temperature-sensitive growth. (A) Strains YN10 (spo20-KC104) and YN12 (WT) were streaked on complete medium (YEA) and incubated at 25 or 37°C for 5 d. (B) Cell morphology at nonpermissive temperatures. YN10 and YN12 were incubated in liquid complete medium (YEL) for 12 h at 25 or 37°C. Cells were fixed and stained with Alexa 568-conjugated phalloidin, calcofluor, and DAPI. Bar, 10 μm.