Figure 9.

Localization of Spo20 during meiosis and sporulation. Homothallic haploid strain YN8-WH cells harboring integrated spo20⁺-HA (A) and TN9 carrying pAL(spo20^G275D-HA) (B) were cultured in SSL-N to induce meiosis. Fixed cells were observed at different stages of meiosis using anti-HA antibody (a–j) and DAPI (a′–d′, f′–i′), as well as observed by phase-contrast microscopy (e′ and j′). a, a′, f, and f′, cells arrested on nitrogen starvation; b, b′, g, and g′, cells in prophase I; c, c′, h, and h′, cells in meiosis I; d, d′, i, and i′, cells in meiosis II; e, e′, j, and j′, sporulated cells. Bars, 10 μm.