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. Author manuscript; available in PMC: 2011 Nov 30.
Published in final edited form as: Biochemistry. 2011 Jul 13;50(32):6920–6932. doi: 10.1021/bi200498q

Figure 2.

Figure 2

Analytical gel filtration of sc-S100A4 proteins. (A) SDS–PAGE of purified wt-S100A4, sc-S100A4–5, and sc-S100A4–10 on a 12% Tris-Tricine gel. Molecular mass markers are indicated on the left. (B) Elution profile of wt-S100A4 (—) and protein standards (---) on a Superdex 75HR column in the presence of EGTA. The inset is a plot of protein standard molecular masses vs elution volume. (C and D) Elution profiles of sc-S100A4–5 and sc-S100A4–10 in the presence of EGTA: wt-S100A4 (—) and sc-S100A4–5 and sc-S100A4–10 (---).