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. Author manuscript; available in PMC: 2011 Nov 30.
Published in final edited form as: Biochemistry. 2011 Jul 13;50(32):6920–6932. doi: 10.1021/bi200498q

Figure 5.

Figure 5

Single-chain S100A4 proteins bind and regulate myosin-IIA assembly. (A) Affinities of S100A4 proteins for myosin-IIA were measured using a fluorescence anisotropy assay. wt-S100A4 (□), sc-S100A4–5 (○), and sc-S100A4–10 (⋄) exhibit similar affinities for myosin-IIA. (B) Promotion of myosin-IIA disassembly. wt-S100A4 (black bars), sc-S100A4–5 (white bars), and sc-S100A4–10 (gray bars) all promote maximal filament disassembly at a 1:1 molar ratio of S100A4 dimer to myosin-IIA dimer. Myosin-IIA disassembly in the absence of S100A4 (hatched bar). Values represent the mean ± standard error of the mean from three independent experiments.