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. Author manuscript; available in PMC: 2011 Nov 30.
Published in final edited form as: Biochemistry. 2011 Jul 13;50(32):6920–6932. doi: 10.1021/bi200498q

Figure 7.

Figure 7

S100A4 single-chain EF-hand mutant proteins exhibit altered Ca2+ affinities. (A) Cartoon of sc-S100A4 EF-hand mutant constructs. Both E33 (EF1) and E74 (EF2) residues in either monomer 1 or monomer 2 were substituted with alanine. Calcium titrations of (B) sc-S100A4–5M1, (C) sc-S100A4–10M1, (D) sc-S100A4–5M2, and (E) sc-S100A4–10M2 in the presence of myosin-IIA and 5,5′Br2-BAPTA. The data represent the average from two or three independent experiments. The insets show the saturation curve representation for the best fit in Caligator.