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. Author manuscript; available in PMC: 2011 Nov 30.
Published in final edited form as: Biochemistry. 2011 Jul 13;50(32):6920–6932. doi: 10.1021/bi200498q

Figure 9.

Figure 9

HCT116 cells require S100A4 for macrophage-dependent invasion. (A) Immunoblot of S100A4 expression in parental and L5 and N3 S100A4−/− HCT116 cells. β-Actin was used as a loading control. (B) Immunoblot of S100A4 protein expression in L5 and N3 S100A4−/− HCT116 cells transfected with wild-type S100A4-GFP, sc-S100A4–5-GFP, or sc-S100A4–5M1-GFP proteins. β-Actin was used as a loading control. (C) Invasion of parental HCT116 cells, L5 and N3 S100A4−/− cells, and N3 S100A4−/− cells transfected with GFP or S100A4-GFP fusion protein in the absence (white bars) or presence (black bars) of macrophages. Values represent the mean ± standard error of the mean for three independent experiments.