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. Author manuscript; available in PMC: 2011 Nov 30.
Published in final edited form as: J Nephrol. 2010 Nov-Dec;23(Suppl 16):S136–S144.

Fig. 3.

Fig. 3

Urinary Klotho protein in chronic kidney disease (CKD) patients normalized to creatinine in spot samples from 13 normal volunteers and 40 CKD patients. For measurement of urinary Klotho protein, 4-ml fresh urine was concentrated to 0.2 ml through Amicon ultra-4 filters with 100-kDa cutoff. Concentrated urines (with identical urine creatinine) along with recombinant murine Klotho (rMKl) protein of known concentration were subject to immunoblot. Klotho protein concentrations in urine samples were quantifed using the rMKl as a standard curve (66). *p<0.05; **p<0.01, vs. normal subjects by 1-way ANOVA followed by Student-Newman-Keuls test.