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Replication kinetics in MT4 cells indicate an increased replication defect of DNA Flap double mutant viruses compared with cPPT or CTS single mutants. MT4 cells were infected with 1 ng p24 of wild-type envelope HIV-1 and virus production was measured at given time points post-infection by performing RT assay on the culture supernatants. Results show the mean of triplicates +/- SEM and are representative of two independent experiments.
