Figure 5.

Quantification of 2-LTR circles and total HIV-1 DNA (Pol) by quantitative PCR shows defects in nuclear import for all DNA Flap mutants. P4-CCR5 cells were infected with wild-type and DNA Flap mutant viruses pseudotyped with VSV-G envelope. (A) Results show the number of 2-LTR circles normalised for pol copy numbers at 24 h post-infection. Non-infected samples did not give rise to amplified products. Cells infected in the presence of Nevirapine served as controls for each virus infection (< 10³ copies for all samples) and were subtracted from each value. Values are represented as a percentage of wild-type and are the mean of 3 independent experiments carried out in duplicate +/- SEM. (B) Results show 2-LTR circles at given time points post-infection, normalised for pol copy number at 24 h post-infection. Values are represented as a percentage of wild-type at 48 h post-infection and are the mean of 2 independent experiments carried out in duplicate +/- SEM.