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. 2011 Nov 30;6(11):e27915. doi: 10.1371/journal.pone.0027915

Figure 6. Luteolin inhibited HIV-1 gene expression independently of viral DNA integration.

Figure 6

A. TZM-bl reporter cells in 12 well culture plates were transfected with HIV-1 plasmid DNA vector expressing GFP, then treated with luteolin (10 µM) or DMSO (Veh). In parallel, TZM-bl cells were infected with VSV-HIV NLENG1 or NLR+E− for 2 h, then treated with luteolin (10 µM) or DMSO for the duration of follow up. At 48 h post-transfection or infection, cells were lysed and assayed for luciferase activity (n = 2). B–C. Two hours after Magi cells were transfected with pHIV NLENG1 (150 ng), luteolin (10 µM) was added to them. After 6 h, transfection medium was replaced with fresh medium containing luteolin for 12 or 24 h. At 48 h post-transfection, cells were monitored for GFP expression. Representative pictures are shown (B). Cell supernatants were collected to measure p24 levels (Cs) (n = 2). *** p<0.001, ### p<0.005.