Figure 4.
Apoptotic and necrotic cells do not compete for recognition by J774A.1 macrophages. Recognition of late apoptotic and necrotic CFDA-labeled DO11.10 targets (A and B, respectively) was assessed in the presence or absence of unlabeled target competitors after incubation for 30 min at 37°C, as in Figure 2. Targets were prepared as in Figure 1. Labeled targets (2 × 105 cells/well) were mixed with unstained apoptotic (▪) or necrotic (□) competitors at the indicated ratios and added to a freshly plated monolayer (2 × 104 cells/well) of J774A.1 macrophages.