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. 2011 Nov 30;6(11):e28131. doi: 10.1371/journal.pone.0028131

Figure 6. ONNV infection ablates the expression of IFN stimulated genes.

Figure 6

(A) Cells were either left untreated (−) or infected with RVFV 13 (+) or ONNV (OV). After 24 h proteins were extracted from cells. Same amount of proteins were subjected to SDS-PAGE followed by a Western blot analysis. Mouse-anti-Mx1/2/3 (MxA), goat-anti-IFIT1/ISG56 (p56) and mouse-anti-actin immunoglobulins were applied at dilutions 1∶1000 followed by a peroxidase labeled goat-anti-mouse or rabbit-anti-goat secondary antibody (1∶20000). In all cell lines infection with ONNV did not induce the expression of MxA and p56 and was less or comparable to untreated cells. (B) To exclude cell clone specific effects additional EidNi and RoNi bat cell cultures were included (EidNi/41.2; EidNi/41 and RoNi/7) as well as a human A549 cell line.