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. 2011 Nov 30;6(11):e27953. doi: 10.1371/journal.pone.0027953

Figure 3. Cross-protection against infections with different influenza virus subtypes.

Figure 3

Six-week-old female BALB/c mice (nā€Š=ā€Š6) were immunized twice with 10 ug of 3M2eC protein plus 2 ug of CT at 2 week intervals via i.n. or s.l., or with 10 ug of 3M2eC protein plus alum by i.d. or i.m.. They were challenged i.n. with 10 LD50 of mouse adapted PR8 strain (H1N1) at 3 weeks (A and B), A/Aquatic Bird/Korea/W81/05 virus (H5N2) at 3 weeks (C and D) or A/Philippine/2/82 (H3N2) virus at 5 weeks (E and F) after the last immunization. Survival rate and the body weight loss were monitored daily after the challenge. The results are expressed as the means+S.D. for the group.