Table 1. Validation of gene expression deregulation through quantitative Real-Time PCR in NIH-3T3 transfectants.
GENE (official gene symbol) | empty | tERG | EWS/ERG | FUS/ERG |
Cdh5 | 1 | (↑) +33.7 | (↑) +8.1 | (↑) +12.4 |
Mmp3 | 1 | (↑) +63.8 | (↑) +79.86 | (↑) +243.9 |
Edn1 | 1 | (↑) +31.9 | (↑) +14.7 | (↑) +21.5 |
Irs1 | 1 | (n) +1.2 | (↑) +2.07 | (↑) +2.49 |
Egln3 | 1 | (↑) +4.6 | (↑) +4.3 | (↑) +2.3 |
Dpysl3 | 1 | (↑) +5.2 | (↑) +7.2 | (↑) +7 |
Cyp1b1 | 1 | (n) +1.1 | (↑) +9.7 | (↑) +5 |
Has2 | 1 | (↓) −1.9 | (↓) −6.9 | (↓) −7.3 |
Ednra | 1 | (↑) +4.8 | (↑) +11.4 | (↑) +42 |
Ccl9 | 1 | (↑) +9 | (↑) +3.1 | (↑) +2.1 |
Gpr149 | 1 | (↑) +5.7 | (↑) +7 | (↑) +5.7 |
Id1 | 1 | (↓) −9.2 | (n) −1.9 | (n) −1.3 |
Significant up- (↑) or down- (↓) regulation obtained by microarray analysis is shown alongside quantitative Real-Time PCR fold change values for a direct comparison. n = no change.