Figure 5. Role of ER remodeling in Ca²⁺ wave propagation.

Cells expressing mCherry-KDEL to visualize ER distribution were injected with caged-IP₃ and Oregon-Green to image Ca²⁺ dynamics. Top panel shows an enlarged region from two cells, one with ER patches and one without. Ca²⁺ waves were induced by uncaging IP₃ within an ROI (red circle) and wave propagation speed was measured. Example images of wave propagation are shown. These experiments were performed on the animal hemisphere (n = 12–13).