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. 2011 Nov 30;6(11):e28240. doi: 10.1371/journal.pone.0028240

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Knierim et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Graphical illustration of the entire workflow. The red arrows depict a measuring and DNA-quantification step. (B) Analysis of fragment lengths by PAGE before (left panel) and after (right panel) removal of small fragments <500 bp with AMPure™ columns. The red boxes depict the desired size range between 600 and 1,000 bp. Neb, nebulization; Son, sonication; Enz, enzymatic fragmentation.