Figure 4. MA region is not required for VLP production.

(A) 293T cells were transfected either with Gag WT, MA mutants (i.e. MAΔ8–132 and MAΔ44–132) or pcDNA3.1 (+) vector. At 48 h post-transfection, both the cell lysates and cell-free supernatants were analyzed on a gradient SDS-PAGE with Coomassie Brilliant Blue staining. (B) In parallel, an IP assay was performed using the anti-p24 antibody on the same samples. Precipitated proteins were run on gradient SDS-PAGE and Gag proteins were analyzed by Western Blotting (WB). The Biochemical experiments shown are representative of at least three independent experiments.