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. 2011 Nov 30;6(11):e28376. doi: 10.1371/journal.pone.0028376

Figure 4. Maturation of newborn cells.

Figure 4

A Distance between the nucleus of different aged cells and the hilus. This distance increased with cell age (p<0.001). Exposure to the Porsolt test reduced the distance in 4 (p = 0.001) and 6 week-old (p = 0.002) cells. B–E Representative images of immunohistochemistry against IdU or CldU (green) and AMPA (blue), in cells counterstained with DAPI (red), showing the relative position of 1 and 6 week-old cells inside the GCL of control animals and those exposed to the Porsolt test. F DCX expression in newborn cells of different ages. The Porsolt test increased the percentage of 4 week-old cells that were also DCX+(p = 0.022), but had no effect in cells of 1, 2 or 3 weeks of age. G–R Representative images of double immunohistochemistry for DCX (red) and IdU or CldU (green). DCX+ and DCX- cells are represented by purple and yellow triangles, respectively. ML: Molecular layer; GCL: Granule cell layer; SGZ: Subgranular zone; H: Hilus. Scale bar = 20 µm.