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. Author manuscript; available in PMC: 2011 Nov 30.
Published in final edited form as: Oncogene. 2010 Nov 22;30(13):1531–1541. doi: 10.1038/onc.2010.533

Figure 3.

Figure 3

RPS6 associates with both wild-type and mutant 5′ TOP messages. (a) Quantitative PCR analysis of the distribution on polysomes of hGH reporter mRNA in HeLa cells transfected with either WT-RPS16-hGH or Cm5-RPS16-hGH plasmids (b) Representative western blot analysis of HeLa cells transfected with either control green florescent protein (GFP), WT-RPS16-hGH or Cm5-RPS16-hGH. A volume of 10 μg of total protein lysates was loaded and the abundance of hGH and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was assessed. (c) Cytoplasmic lysates from MCF-7 and HeLa cell lines were used for immunoprecipitation assays, using anti-RPS6 antibody. RNA was isolated and reverse transcription followed by qPCR was performed to measure abundance of RNA. Graph represents the mean from three independent assays.