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. Author manuscript; available in PMC: 2011 Nov 30.
Published in final edited form as: Phys Med Biol. 2010 Jun 24;55(14):3947–3957. doi: 10.1088/0031-9155/55/14/001

Figure 1.

Figure 1

Schematic of optical-CT/ECT systems for imaging cleared tissue samples. The system makes use of a telecentric lens to reduce the number of scattered photons and to acquire accurate line integrals. Dashed lines represent possible light rays not traveling parallel to the optical axis and therefore rejected by the lens. (A) Optical-CT mode. A collimated light source is used to transmit a parallel beam through the sample and filter to acquire reconstructions at the desired wavelength. (B) Optical-ECT mode. Two excitation sources enter the tissue anti-parallel to each other and perpendicular to the image axis of the telecentric lens.