Biochemical characterization of anti-ALCAM CysDb. a, SDS-PAGE analysis of anti-ALCAM CysDb and, for comparison, anti-HER2 CysDb (1, anti-HER2 CysDb; 2, anti-ALCAM CysDb; M, marker; 3, reduced anti-HER2 CysDb; 4, reduced anti-ALCAM CysDb). b, Size exclusion chromatography elution profile of anti-ALCAM CysDb on Superdex 75.