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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Mol Imaging Biol. 2012 Jun;14(3):336–347. doi: 10.1007/s11307-011-0500-8

Figure 3.

Figure 3

Functional characterization of anti-ALCAM CysDb. a, Evaluation of binding specificity by flow cytometry. Left, HPAF-II; middle, BxPC-3; right, C6. Filled peak, cells only. Solid line, cells incubated with anti-ALCAM CysDb, followed by mouse anti-Penta-His antibody and PE-conjugated goat anti-mouse antibody. Dashed line, cells incubated with only mouse anti-Penta-His antibody and PE-conjugated goat anti-mouse antibody. b, Evaluation of binding to cultured cells by immunofluorescence. Top panels, HPAF-II; middle panels, BxPC-3; bottom panels, C6. Left column, DAPI staining. Middle column, Alexa Fluor 647-anti-ALCAM CysDb staining. Right column, overlay. c, Determination of KD by flow cytometry.